What is immunosignature analysis and how is it different from traditional approaches?
Immunosignature analysis examines the entire pattern of immune responses across hundreds or thousands of antigens simultaneously. Unlike traditional approaches that test 5-10 selected antigens, we use peptide and protein microarray technology combined with machine learning to screen a depurated selection of antigens revealing which antigens could drive protective immunity in complex vaccines and bacterial infections. This is critical when dealing with whole-cell vaccines or pathogens with 2,000+ potential antigens.
What immunosignature services do you offer?
We specialize in three core areas:
Complex Vaccine Immunoprofiling – Identifying antigen combinations in multi-component and whole-cell vaccines that could generate protective responses
Bacterial Infection Immunosignature Analysis – Mapping immune responses to complex bacterial pathogens with hundreds of potential antigens
Machine Learning-Enhanced Data Analysis – Our advanced ML approaches identify non-obvious antigen interactions and predict synergistic combinations
All services include comprehensive bioinformatics analysis and detailed data packages to support your research and hypothesis refinement.
How long does an immunosignature project take?
Our microarrays are not off-the-shelf. Each project uses a panel that is designed and produced specifically for your study. Because everything is tailor-made, including array design, printing, QC, and downstream analysis; timelines depend on the complexity of the work
If you tell us what you need, we can give you a realistic timeframe based on your design and analytical requirements.
What sample volume and type do you need for peptide/protein microarray analysis?
We work with minimal sample volumes to preserve your precious research specimens:
Serum/Plasma: 10-20 μL per sample to test
Purified antibodies: 10-50 μg depending on affinity
Cell culture supernatants: 100-200 μL
We accept serum, plasma (derived from whole blood), CSF, and purified antibody samples. Samples can be fresh or frozen. We'll provide detailed sample preparation guidelines upon project initiation.
What quality standards do you follow?
We follow rigorous quality control protocols designed for research discovery:
• Complete documentation with audit trails
• Standardized microarray protocols with quality controls
• Statistical analysis reports with raw data files in multiple formats
• Detailed bioinformatics analysis to guide further research
Important: Our services are designed to support discovery research and hypothesis generation. If you plan to use our data for regulatory submissions or clinical applications, you'll need to validate findings through your own regulatory-compliant processes or with additional GLP-compliant services as appropriate.
How does immunosignature analysis compare to traditional antigen screening?
Immunosignature analysis with peptide microarrays offers several key advantages:
Scale: Screen entire pathogen proteomes (1,000-5,000+ antigens) vs. 5-10 with traditional methods
Unbiased discovery: Identifies unexpected protective antigens you wouldn't have selected
Sample efficiency: Uses 10-20 μL vs. mL required for multiple ELISAs
Combination analysis: ML models reveal which antigens work synergistically
Cost-effectiveness: Dramatically lower cost per antigen tested
While targeted assays remain valuable for validation, immunosignature analysis is unmatched for complex vaccine antigen discovery and optimization.
Can you help with complex vaccine development projects?
Absolutely. Immunosignature analysis is essential for complex vaccine development. We support:
• Multi-component vaccine optimization (which antigens to include/exclude)
• Whole-cell vaccine characterization (identifying protective antigen combinations)
• Bacterial vaccine antigen selection from entire proteomes
• Combination vaccine formulation decisions
• Identifying synergistic vs. redundant antigens
• Diagnostic antigen panel selection
Our ML-powered analysis helps you stop guessing which antigens work and start making data-driven formulation decisions.
How does your machine learning approach improve immunosignature analysis?
Traditional immunosignature analysis identifies reactive antigens but struggles with complexity: Which antigens work synergistically? Which combinations predict protection? How do cross-reactive responses complicate interpretation?
Our machine learning models:
• Identify antigen combinations that correlate with clinical outcomes
• Detect non-linear interactions between antigens
• Predict which antigen sets optimize vaccine efficacy
• Distinguish protective vs. non-protective response patterns
• Account for cross-reactivity and immune interference
We transform complex data into actionable insights about antigen selection and vaccine optimization.
How are you different from other immunosignature providers?
We use similar peptide and protein microarray technology platforms but enhance the analysis with advanced machine learning. Think of us as bringing AI-powered interpretation to immunosignature profiling.
Traditional immunosignature output: "Here are 347 reactive antigens"
ImmunoSig ML-enhanced output: "Here are the 6 antigen combinations that correlate with protection, ranked by predictive power, with synergy analysis"
Key differentiators:
1. ML-powered combination analysis: Identifies which antigen sets work together
2. Complex vaccine focus: We specialize in multi-antigen systems, not single-protein characterization
3. Vaccine development expertise: Our team has direct experience developing bacterial vaccines
How do I get started with an immunoprofiling project?
Getting started is simple:
1. Initial Consultation: Contact us to discuss your project goals, timeline, and sample availability (15-30 min call)
2. Proposal: We'll provide a detailed technical proposal including array design, timeline, deliverables, and pricing within 3-5 business days
3. Project Kickoff: Once approved, we'll send sample shipment instructions and initiate array production
4. Results Delivery: Receive comprehensive analysis reports with raw data, statistical analysis, and publication-quality figures
Contact us today to schedule your free consultation.
What types of vaccines and pathogens do you work with?
Our immunosignature services are especially valuable for complex systems:
• Whole-cell bacterial vaccines (pertussis, typhoid, cholera)
• Multi-component combination vaccines
• TB vaccine candidates with multiple antigens
• Bacterial infection immunology (Streptococcus , Staphylococcus, E. coli, etc.)
• Complex microorganisms (parasites, fungi)
• Veterinary vaccines with undefined protective antigens
• Diagnostic antigen discovery
If you're asking "which of these 500+ antigens actually matter?" - that's where we excel.
Can you handle confidential or proprietary sequences?
Yes. Confidentiality is paramount. We routinely work with:
• Proprietary therapeutic antibody sequences
• Unpublished vaccine antigens
• Novel biologics under development
All projects are covered by comprehensive confidentiality agreements. We can accommodate special security requirements for highly sensitive programs.
